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KMID : 1007520180270030829
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Food Science and Biotechnology
2018 Volume.27 No. 3 p.829 ~ p.835
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Effect of glutamine on heat-shock protein beta 1 (HSPB1) expression during myogenic differentiation in bovine embryonic fibroblast cells
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Kim Young-Shin
Lee Jae-Sung
Lee Yoon-Seok
Kim Won-Seob
Peng Dong-Qiao
Bae Mun-Hee
Jo Yong-Ho
Baik Myung-Gi
Lee Hong-Gu
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Abstract
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The objective of this study was to examine the effects of glutamine on heat-shock protein beta 1 (HSPB1) expression in bovine embryonic fibroblast cells during myogenesis. First, to elucidate the role of glutamine on HSPB1 expression during myogenesis, we treated with glutamine in myogenic lineage determinant (MyoD) over-expressed bovine embryonic fibroblast cells (BEFS-MyoD cells). Second, knockdown of HSPB1 using small interference RNA was performed to evaluate whether muscle development by glutamine is dependent on HSPB1 in BEFS-MyoD cells. As a result, glutamine promoted the mRNA level of HSPB1, Myogenin, Desmin, and mTOR as well as myotube formation, and protein synthesis (p < 0.05). The inhibition of HSPB1 expression during myogenesis has shown to repress the expression of myogenic marker genes (MyoD, Myogenin, Desmin) (p < 0.01), formation of myotubes and protein synthesis (p < 0.05). According to the results, it is concluded that glutamine regulates HSPB1 expression during myogenesis.
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KEYWORD
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Glutamine, HSPB1, Bovine embryonic fibroblast cell, Myogenic differentiation, Protein synthesis
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